Prelab Brief
To prepare a temporary mount of an onion peel and observe plant cell structure under a microscope.
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Theory
Examine the structure of a plant cell with interactive labelled diagrams. To prepare a temporary mount of an onion peel and observe plant cell structure under a microscope.
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Formula
Principle: To prepare a temporary mount of an onion peel and observe plant cell structure under a microscope.
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Aim
To prepare a temporary mount of an onion peel and observe plant cell structure under a microscope.
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Apparatus
- Microscope
- Onion
- Glass slides and cover slips
- Forceps and needle
- Safranin stain
- Dropper and blotting paper
- Watch glass
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Procedure
- Peel a thin layer from the inner surface of an onion scale.
- Place the peel on a clean glass slide.
- Add a drop of safranin stain and wait 2 minutes.
- Place a cover slip gently, avoiding air bubbles.
- Observe under low power (10x) then high power (40x).
- Identify cell wall, cell membrane, nucleus, and vacuole.
Pre-Lab Questions
What is the main aim of the Plant Cell Explorer Experiment?
Which principle is demonstrated in the Plant Cell Explorer Experiment?
What apparatus is required for this experiment?
Why is Microscope used in this experiment?
What formula or relationship is verified in this experiment?
What precaution should be followed while performing this experiment?
What conclusion can be drawn from the Plant Cell Explorer Experiment?