Prelab Brief
To demonstrate that CO₂ is released during respiration by germinating seeds.
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Theory
Demonstrate aerobic and anaerobic respiration using interactive simulations. To demonstrate that CO₂ is released during respiration by germinating seeds.
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Formula
Principle: To demonstrate that CO₂ is released during respiration by germinating seeds.
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Aim
To demonstrate that CO₂ is released during respiration by germinating seeds.
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Apparatus
- Conical flask
- Germinating seeds (gram/pea)
- KOH solution (to absorb CO₂)
- Lime water (Ca(OH)₂)
- Delivery tube
- Cotton plug
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Procedure
- Place germinating seeds in a conical flask.
- Connect the flask to a test tube containing lime water via delivery tube.
- Seal the flask with a cotton plug.
- Leave for 1-2 hours.
- Observe the lime water turning milky → confirms CO₂ release.
- Repeat with boiled (dead) seeds as control — no change in lime water.
Pre-Lab Questions
What is the main aim of the Respiration Lab Experiment?
Which principle is demonstrated in the Respiration Lab Experiment?
What apparatus is required for this experiment?
Why is Conical flask used in this experiment?
What formula or relationship is verified in this experiment?
What precaution should be followed while performing this experiment?
What conclusion can be drawn from the Respiration Lab Experiment?