Biology Experiments / Stomata Exploration

Stomata Exploration Experiment

Observe stomatal pores on leaf epidermis under a virtual microscope.

Biology Class 9-10 Plant-physiology Intermediate
Prelab Brief
To prepare a temporary mount of a leaf peel and observe stomata under a microscope.
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Theory

Observe stomatal pores on leaf epidermis under a virtual microscope. To prepare a temporary mount of a leaf peel and observe stomata under a microscope.

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Formula

Principle: To prepare a temporary mount of a leaf peel and observe stomata under a microscope.

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Aim

To prepare a temporary mount of a leaf peel and observe stomata under a microscope.

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Apparatus

  • Microscope
  • Fresh leaf (Tradescantia / Rhoeo)
  • Glass slide and cover slip
  • Safranin stain
  • Needle and forceps
  • Dropper and blotting paper
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Procedure

  1. Fold the leaf and gently peel the lower epidermis.
  2. Place the peel on a slide with a drop of water.
  3. Add safranin stain, wait 2 minutes, then place cover slip.
  4. Observe under 10x then 40x magnification.
  5. Identify guard cells, stomatal pore, and epidermal cells.
  6. Count stomata in the field of view.
Pre-Lab Questions
What is the main aim of the Stomata Exploration Experiment?
Which principle is demonstrated in the Stomata Exploration Experiment?
What apparatus is required for this experiment?
Why is Microscope used in this experiment?
What formula or relationship is verified in this experiment?
What precaution should be followed while performing this experiment?
What conclusion can be drawn from the Stomata Exploration Experiment?

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