Prelab Brief
To demonstrate that water and dissolved substances are transported upward through xylem vessels in plants using a coloured dye experiment.
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Theory
Demonstrate how water travels through xylem vessels using a coloured dye and celery / white flower stem. To demonstrate that water and dissolved substances are transported upward through xylem vessels in plants using a coloured dye experiment.
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Formula
Principle: To demonstrate that water and dissolved substances are transported upward through xylem vessels in plants using a coloured dye experiment.
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Aim
To demonstrate that water and dissolved substances are transported upward through xylem vessels in plants using a coloured dye experiment.
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Apparatus
- Fresh celery stalk with leaves (or white carnation)
- Glass beaker / jar (250 mL)
- Food colouring / coloured dye (red, blue, or green)
- Sharp knife / blade
- Water
- Ruler
- Clock / stopwatch
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Procedure
- Select a fresh celery stalk or white flower stem and make a fresh diagonal cut at the base.
- Fill the beaker with water and add several drops of food colouring. Stir well.
- Place the freshly cut stem into the coloured water immediately.
- Leave the setup in a bright, warm location for 2–4 hours (or overnight for deeper colour).
- Observe gradual colour change in the stem, leaves, and petals.
- Optionally, cut the stem cross-sectionally to observe coloured xylem vessels.
Pre-Lab Questions
What is the main aim of the Water Transport in Plants (Xylem) Experiment?
Which principle is demonstrated in the Water Transport in Plants (Xylem) Experiment?
What apparatus is required for this experiment?
Why is Fresh celery stalk with leaves (or white carnation) used in this experiment?
What formula or relationship is verified in this experiment?
What precaution should be followed while performing this experiment?
What conclusion can be drawn from the Water Transport in Plants (Xylem) Experiment?