Prelab Brief
To separate the components of a mixture (like dyes in black ink) based on their different solubilities in a solvent.
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Theory
Separate the different colored dyes present in black ink using a solvent and filter paper. To separate the components of a mixture (like dyes in black ink) based on their different solubilities in a solvent.
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Formula
Principle: To separate the components of a mixture (like dyes in black ink) based on their different solubilities in a solvent.
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Aim
To separate the components of a mixture (like dyes in black ink) based on their different solubilities in a solvent.
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Apparatus
- Strip of filter paper (chromatography paper)
- Capillary tube
- Beaker/Glass jar with lid
- Paper clip / Glass rod
- Black ink spot
- Solvent (Water or alcohol)
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Procedure
- Draw a pencil line about 3 cm from the bottom of the filter paper strip.
- Put a small drop of black ink in the center of the pencil line.
- Pour a small amount of solvent into the jar.
- Suspend the paper strip in the jar so the tip is in the solvent, but the ink spot is above the solvent level.
- Cover the jar and let the solvent rise up the paper.
- Observe the separation of colors as the solvent moves upward.
Pre-Lab Questions
What is the main aim of the Paper Chromatography Experiment?
Which principle is demonstrated in the Paper Chromatography Experiment?
What apparatus is required for this experiment?
Why is Strip of filter paper (chromatography paper) used in this experiment?
What formula or relationship is verified in this experiment?
What precaution should be followed while performing this experiment?
What conclusion can be drawn from the Paper Chromatography Experiment?